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recombinant growth differentiation factor 11 gdf11  (R&D Systems)


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    R&D Systems recombinant growth differentiation factor 11 gdf11
    Figure 5 Myostatin-specific inhibition by myostatin-b. HeLa cells cotransfected with Luc and Gal plasmids were treated with recombinant myostatin at concentrations of 0, 20, 40 and 60 ng/mL (A); recombinant growth differentiation factor 11 <t>(GDF11)</t> at concentrations of 0, 10, 20 and 30 ng/mL (B); recombinant transforming growth factor β1 (TGF-β1) at concentrations of 0, 0.5, 1 and 2 ng/mL (C); and activin A at concentrations of 0, 10, 20 and 30 ng/mL (D). In addition, the myostatin-b plasmid was cotransfected into each treated cell. The results of luciferase activity are shown. Myostatin-b inhibited myostatin signalling induced only by recombinant myostatin but not by recombinant GDF11, recombinant TGF-β1 or recombinant activin A (red bars). The data are expressed as the mean ± SEM of three independent experiments. **P < 0.01, ***P < 0.001. RLU, relative luminescence unit.
    Recombinant Growth Differentiation Factor 11 Gdf11, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+growth+differentiation+factor+11+gdf11/Recombinant+Human%2FMouse%2FRat+GDF-11%2FBMP-11+Protein/pm37582652-88-19-26
    Average 92 stars, based on 44 article reviews
    recombinant growth differentiation factor 11 gdf11 - by Bioz Stars, 2026-10
    92/100 stars

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    1) Product Images from "A novel splice variant of the human MSTN gene encodes a myostatin-specific myostatin inhibitor."

    Article Title: A novel splice variant of the human MSTN gene encodes a myostatin-specific myostatin inhibitor.

    Journal: Journal of cachexia, sarcopenia and muscle

    doi: 10.1002/jcsm.13314

    Figure 5 Myostatin-specific inhibition by myostatin-b. HeLa cells cotransfected with Luc and Gal plasmids were treated with recombinant myostatin at concentrations of 0, 20, 40 and 60 ng/mL (A); recombinant growth differentiation factor 11 (GDF11) at concentrations of 0, 10, 20 and 30 ng/mL (B); recombinant transforming growth factor β1 (TGF-β1) at concentrations of 0, 0.5, 1 and 2 ng/mL (C); and activin A at concentrations of 0, 10, 20 and 30 ng/mL (D). In addition, the myostatin-b plasmid was cotransfected into each treated cell. The results of luciferase activity are shown. Myostatin-b inhibited myostatin signalling induced only by recombinant myostatin but not by recombinant GDF11, recombinant TGF-β1 or recombinant activin A (red bars). The data are expressed as the mean ± SEM of three independent experiments. **P < 0.01, ***P < 0.001. RLU, relative luminescence unit.
    Figure Legend Snippet: Figure 5 Myostatin-specific inhibition by myostatin-b. HeLa cells cotransfected with Luc and Gal plasmids were treated with recombinant myostatin at concentrations of 0, 20, 40 and 60 ng/mL (A); recombinant growth differentiation factor 11 (GDF11) at concentrations of 0, 10, 20 and 30 ng/mL (B); recombinant transforming growth factor β1 (TGF-β1) at concentrations of 0, 0.5, 1 and 2 ng/mL (C); and activin A at concentrations of 0, 10, 20 and 30 ng/mL (D). In addition, the myostatin-b plasmid was cotransfected into each treated cell. The results of luciferase activity are shown. Myostatin-b inhibited myostatin signalling induced only by recombinant myostatin but not by recombinant GDF11, recombinant TGF-β1 or recombinant activin A (red bars). The data are expressed as the mean ± SEM of three independent experiments. **P < 0.01, ***P < 0.001. RLU, relative luminescence unit.

    Techniques Used: Inhibition, Recombinant, Plasmid Preparation, Luciferase, Activity Assay

    Related Articles

    Recombinant:

    Article Title: A novel splice variant of the human MSTN gene encodes a myostatin-specific myostatin inhibitor.
    Article Snippet: Myostatin signal activity was assayed using the SBE4-Luc luciferase reporter gene containing the Smad-binding sequence (CAGA) (#16495, Addgene, Watertown, MA, USA).26 Rhabdomyosarcoma or HeLa cells were seeded at a density of 2 × 105 cells per well in 12-well plates for 24 h before transfection with the SBE4-Luc luciferase reporter gene and pSV-β-galactosidase control vector (#E1081, Promega, Madison, WI, USA) and either the empty vector (pcDNA3.1(+)) or pcDNA3.1(+) harbouring pro-myostatin and myostatin-b sequences. .. After 24 h, the medium was replaced with serum-free RPMI 1640 or DMEM containing recombinant myostatin (788-G8, R&D Systems), recombinant growth differentiation factor 11 (GDF11) (1958-GD, R&D Systems), recombinant TGF-β1 (240-B/CF, R&D Systems) or recombinant activin A (338-AC/CF, R&D Systems). .. After an additional 24 h of culture, the cells were processed, and their luciferase and β-galactosidase activities were analysed using a luciferase reporter assay system and a β-galactosidase enzyme assay system, respectively (Promega).

    Article Title: A novel splice variant of the human MSTN gene encodes a myostatin‐specific myostatin inhibitor
    Article Snippet: Rhabdomyosarcoma or HeLa cells were seeded at a density of 2 × 10 5 cells per well in 12‐well plates for 24 h before transfection with the SBE4‐Luc luciferase reporter gene and pSV‐β‐galactosidase control vector (#E1081, Promega, Madison, WI, USA) and either the empty vector (pcDNA3.1(+)) or pcDNA3.1(+) harbouring pro‐myostatin and myostatin‐b sequences. .. After 24 h, the medium was replaced with serum‐free RPMI 1640 or DMEM containing recombinant myostatin (788‐G8, R&D Systems), recombinant growth differentiation factor 11 (GDF11) (1958‐GD, R&D Systems), recombinant TGF‐β1 (240‐B/CF, R&D Systems) or recombinant activin A (338‐AC/CF, R&D Systems). .. After an additional 24 h of culture, the cells were processed, and their luciferase and β‐galactosidase activities were analysed using a luciferase reporter assay system and a β‐galactosidase enzyme assay system, respectively (Promega).



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    recombinant growth differentiation factor 11 gdf11  (R&D Systems)
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    R&D Systems recombinant growth differentiation factor 11 gdf11
    Figure 5 Myostatin-specific inhibition by myostatin-b. HeLa cells cotransfected with Luc and Gal plasmids were treated with recombinant myostatin at concentrations of 0, 20, 40 and 60 ng/mL (A); recombinant growth differentiation factor 11 <t>(GDF11)</t> at concentrations of 0, 10, 20 and 30 ng/mL (B); recombinant transforming growth factor β1 (TGF-β1) at concentrations of 0, 0.5, 1 and 2 ng/mL (C); and activin A at concentrations of 0, 10, 20 and 30 ng/mL (D). In addition, the myostatin-b plasmid was cotransfected into each treated cell. The results of luciferase activity are shown. Myostatin-b inhibited myostatin signalling induced only by recombinant myostatin but not by recombinant GDF11, recombinant TGF-β1 or recombinant activin A (red bars). The data are expressed as the mean ± SEM of three independent experiments. **P < 0.01, ***P < 0.001. RLU, relative luminescence unit.
    Recombinant Growth Differentiation Factor 11 Gdf11, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+growth+differentiation+factor+11+gdf11/Recombinant+Human%2FMouse%2FRat+GDF-11%2FBMP-11+Protein/pm37582652-88-19-26
    Average 92 stars, based on 1 article reviews
    recombinant growth differentiation factor 11 gdf11 - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 5 Myostatin-specific inhibition by myostatin-b. HeLa cells cotransfected with Luc and Gal plasmids were treated with recombinant myostatin at concentrations of 0, 20, 40 and 60 ng/mL (A); recombinant growth differentiation factor 11 (GDF11) at concentrations of 0, 10, 20 and 30 ng/mL (B); recombinant transforming growth factor β1 (TGF-β1) at concentrations of 0, 0.5, 1 and 2 ng/mL (C); and activin A at concentrations of 0, 10, 20 and 30 ng/mL (D). In addition, the myostatin-b plasmid was cotransfected into each treated cell. The results of luciferase activity are shown. Myostatin-b inhibited myostatin signalling induced only by recombinant myostatin but not by recombinant GDF11, recombinant TGF-β1 or recombinant activin A (red bars). The data are expressed as the mean ± SEM of three independent experiments. **P < 0.01, ***P < 0.001. RLU, relative luminescence unit.

    Journal: Journal of cachexia, sarcopenia and muscle

    Article Title: A novel splice variant of the human MSTN gene encodes a myostatin-specific myostatin inhibitor.

    doi: 10.1002/jcsm.13314

    Figure Lengend Snippet: Figure 5 Myostatin-specific inhibition by myostatin-b. HeLa cells cotransfected with Luc and Gal plasmids were treated with recombinant myostatin at concentrations of 0, 20, 40 and 60 ng/mL (A); recombinant growth differentiation factor 11 (GDF11) at concentrations of 0, 10, 20 and 30 ng/mL (B); recombinant transforming growth factor β1 (TGF-β1) at concentrations of 0, 0.5, 1 and 2 ng/mL (C); and activin A at concentrations of 0, 10, 20 and 30 ng/mL (D). In addition, the myostatin-b plasmid was cotransfected into each treated cell. The results of luciferase activity are shown. Myostatin-b inhibited myostatin signalling induced only by recombinant myostatin but not by recombinant GDF11, recombinant TGF-β1 or recombinant activin A (red bars). The data are expressed as the mean ± SEM of three independent experiments. **P < 0.01, ***P < 0.001. RLU, relative luminescence unit.

    Article Snippet: After 24 h, the medium was replaced with serum-free RPMI 1640 or DMEM containing recombinant myostatin (788-G8, R&D Systems), recombinant growth differentiation factor 11 (GDF11) (1958-GD, R&D Systems), recombinant TGF-β1 (240-B/CF, R&D Systems) or recombinant activin A (338-AC/CF, R&D Systems).

    Techniques: Inhibition, Recombinant, Plasmid Preparation, Luciferase, Activity Assay